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Which chromatographic technique uses a solid stationary phase?
- A Gas chromatography
- B Liquid chromatography
- C Ion-exchange chromatography
- D Thin-layer chromatography
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Thin-layer chromatography (TLC) is the chromatographic technique that uses a solid stationary phase. In TLC, the stationary phase is typically a thin layer of an adsorbent material, such as silica gel or alumina, coated onto a flat, inert support, such as a glass plate. The sample mixture is spotted onto the stationary phase, and a solvent, called the mobile phase, is allowed to move up the plate via capillary action. As the solvent moves, it carries the components of the sample mixture along, and separation occurs based on their different affinities for the stationary phase.