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Which technique is commonly used for the separation and analysis of proteins and nucleic acids?

  • A Gas chromatography
  • B Liquid chromatography
  • C Thin-layer chromatography
  • D Size-exclusion chromatography
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Liquid chromatography, particularly High-Performance Liquid Chromatography (HPLC), is a versatile technique that enables efficient separation and analysis of complex mixtures of proteins, nucleic acids, and other biomolecules. It offers high resolution, sensitivity, and reproducibility, making it a popular choice in various fields, including biochemistry, molecular biology, and proteomics. While the other chromatography techniques mentioned can also be used for separation and analysis, liquid chromatography is most suited for the analysis of proteins and nucleic acids due to its ability to handle delicate biomolecules without significant loss of biological activity or structural integrity.

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