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Which type of chromatography separates compounds based on their affinity for a stationary phase?
- A Size-exclusion chromatography
- B Ion-exchange chromatography
- C Affinity chromatography
- D Gas chromatography
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Affinity chromatography is the type of chromatography that separates compounds based on their affinity for a stationary phase. In this technique, the stationary phase is designed to specifically interact with the target compound or analyte. The sample mixture is passed through the column containing the stationary phase, and the target compound binds selectively to the stationary phase due to specific interactions such as ligand-receptor binding, antibody-antigen interactions, or enzyme-substrate interactions. This allows for the isolation and purification of the target compound from complex mixtures.